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Involvement of a transforming-growth-factor-β-like molecule in tumor-cell-derived inhibition of nitric-oxide synthesis in cerebral endothelial cells

✍ Scribed by Jun-Ichi Murata; Sally Betz Corradin; Emanuela Felley-bosco; Lucienne Juillerat-Jeanneret

Publisher
John Wiley and Sons
Year
1995
Tongue
French
Weight
797 KB
Volume
62
Category
Article
ISSN
0020-7136

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✦ Synopsis

Nitric oxide (NO) has been shown to exert cytotoxic effects on tumor cells. W e have reported that EC2 I 9 cells, a rat-brainmicrovessel-derived endothelial cell line, produced NO through cytokine-inducible NO synthase (iNOS), the induction of which was significantly decreased by (a) soluble factor(s) secreted by DHDIPROb, an invasive sub-clone of a rat colon-carcinoma cell line. In this study, the DHDiPROb cell-derived NO-inhibitory factor was characterized. Northern-blot analysis demonstrated that the induction of iNOS mRNA in cytokine-activated EC219 cells was decreased by PROb-cell-conditioned medium. When DHDiPROb cell supernatant was fractionated by affinity chromatography using Con A-Sepharose or heparin-Sepharose, the NO-inhibitory activity was found only in Con A-unbound or heparin-unbound fractions, respectively, indicating that the PROb-derived inhibitory factor was likely to be a nonglycosylated and non-heparin-binding molecule. Pre-incubation of DHDiPROb-cell supernatant with anti-TGF-P neutralizing antibody completely blocked the DHDiPROb-derived inhibition of N O production by EC219 cells. Addition of exogenous TGF-pi dose-dependently inhibited NO release by EC2 I 9 cells. The presence of active TGF-f3 in the DHDiPROb cell supernatant was demonstrated using a growth-inhibition assay. Moreover, heat treatment of medium conditioned by the less invasive DHDiREGb cells, which constitutively secreted very low levels of active TGF-p, increased both TGF-f3 activity and the ability to inhibit N O production in EC219 cells. Thus, DHDiPROb coloncarcinoma cells inhibited NO production in EC219 cells by secreting a factor identical or very similar to TGF-P.

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