InVivo Release of 14C-Labelled Phenolic Groups from Intact Dietary Spinach Cell Walls During Passage Through the Rat Intestine
✍ Scribed by Buchanan, Callum J; Wallace, Graham; Fry, Stephen C; Eastwood, Martin A
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 918 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Feruloyl and p-coumaroyl groups in spinach cell walls (CW) were labelled using [14C]cinnamic acid and fed to rats. In the caecum and colon, ferulic acid (FA) and p-coumaric acid (PCA) were released from the CW. Few feruloyl or coumaroyl groups remained in the CW to be excreted in faeces, and thus the presence of simple phenol-sugar esters provided little protection of the polysaccharides to enzymic attack. Some oxidatively coupled phenols were also released but a portion remained in the CW. The oxidatively coupled phenols accumulated in the gut whereas the FA and PCA were absorbed by the rat. Thus enzyme-resistant fragments, containing oxidatively coupled phenols (and possibly sugar residues), may survive microbial attack by rat intestinal bacteria.