Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) -jeffamine® hydrogel beads

✍ Scribed by Guohua Chen; Leen Van Der Does; Adriaan Bantjes

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 747 KB
• Volume: 48
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1993.070480707

✦ Synopsis

Hydrogel-like polymers with reactive cyclic carbonate ( CCA) groups have been obtained by the cross-linking reaction of poly(viny1ene carbonate) (PVCA) with Jeffamines@. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.