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Investigations on the resolving power of hydroxyapatite columns

✍ Scribed by Tsutomu Kawasaki; Giorgio Bernardi

Publisher
Wiley (John Wiley & Sons)
Year
1970
Tongue
English
Weight
527 KB
Volume
9
Category
Article
ISSN
0006-3525

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✦ Synopsis

Synopsis

An investigation on the resolving power of hydroxyapatite columns is reported. The macromolecules chosen for this investigation have been five proteins endowed with a rigid structure and of different sizes : cytochrome c, lysozyme, B-lactoglobulin A, collagen, and T2 phage. The following points have been investigated in detail: (1) the dependence of the elution molarity upon column length and slope of the gradient; (2) the dependence of the elution molarity and the width of the protein peak upon the load and the presence of other chromatographic components; (3) the optimal conditions for the resolution of macromolecules having the same size or different sizes.

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