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Investigations of subtilisin (Novo type) immobilization on cellulose by means of various methods of support activation

✍ Scribed by T. Trzmiel; E. Galas; M. Fortak

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 294 KB
Volume: 14
Category: Article
ISSN: 0138-4988
DOI: 10.1002/abio.370140215

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✦ Synopsis

The immobilization of subtilisin, Nouo type, on WHATMAN cellulose activated by the glutaraldehyde, diisocyanate, diazo-coupling, and S-triazine method, and on a dialdehyde derivative of cellulose has been studied. The best results of proteinase immobilization were achieved when cellulose was activated by hexamethylene diisocyanate. The preparations of a proteolytic activity of 70 PU/g of support and of an esterase activity of 2.3 EU/g of support were obtained with 14% and 38% yield, respectively.

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