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Investigation on False Peak Phenomena in On-line Sweeping Technique in MEKC

✍ Scribed by Geng-Liang Yang; Bao-Hui Li; De-Xian Wang; Yi Chen

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
289 KB
Volume
20
Category
Article
ISSN
0256-7660

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✦ Synopsis

Abstract

In this paper, several {actors that could lead to the appearance of false peak were investigated by using on‐line sample sweeping technique under different experimental conditions. The tested analytes were buflomedil hydrochloride, ephedrine hydrochloride, benzyl alcohol, vanillin, p‐hydroxybenzaldehyde and m‐methylphenol. Results showed mat among the six compounds, three of them, i.e., buflomedil hydrochloride, ephedrine hydrochloride and benzyl alcohol will cause false peaks to appear when sample injection time is long, sodium dodecyl sulfate (SDS) concentration is high and there is pH gradient between cathode and anode. In order to avoid the appearance of false peak, the pH gradient should be avoided.

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✍ Jun Cao; Bin Li; Yan-Xu Chang; Ping Li 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 266 KB

## Abstract Conventionally, neutral compounds cannot be separated by nonionic micelle capillary electrophoresis. In this report, the development of a novel on‐line preconcentration technique combining dual sweeping based on complexation and organic solvent field enhancement is applied to the sensit