Investigation of vaccinia virus DNA replication employing a conditional lethal mutant defective in DNA
โ Scribed by Beatriz G.T. Pogo; Elchonon M. Berkowitz; Samuel Dalest
- Book ID
- 118367706
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 755 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0042-6822
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A temperature-sensitive mutant of E. coli with a defective DNA gyrase B subunit has been obtained. The mutation is expressed in the thermolability of DNA gyrase in vitro and in DNA relaxation in vivo. DNA replication in the mutant does not stop under non-permissive conditions; its rate gradually fal
Mutants of Escherichia coli completely deficient in RNase H activity were isolated by inserting transposon Tn3 into the structural gene for RNase H, rnh, and its promoter. These rnh- mutants exhibited the following phenotypes; (1) the mutants grew fairly normally, (2) rnh- cells could be transformed