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Investigation of the functional role of active site loop II in a hypoxanthine phosphoribosyltransferase

✍ Scribed by Christian C Lee; Francisco J. Medrano; Sydney P Craig III; Ann E Eakin


Book ID
117617824
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
317 KB
Volume
1537
Category
Article
ISSN
0925-4439

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Functional roles for amino acids in acti
✍ Francisco J Medrano; Mary A Wenck; Ann E Eakin; Sydney P Craig III πŸ“‚ Article πŸ“… 2003 πŸ› Elsevier Science 🌐 English βš– 350 KB

A flexible loop of amino acids (loop II) closes over the active site of hypoxanthine phosphoribosyltransferase (HPRT) as the enzyme approaches the transition state [Biochemistry 37 (1998) 17120]. Formerly, the deletion of much of loop II from the HPRT of Trypanosoma cruzi resulted in a 2-3 order of