Investigation of the batch injection analysis technique with amperometric biocatalytic electrodes using a modified small-volume cell
✍ Scribed by A. Amine; J.-M. Kauffmann; G. Palleschi
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 451 KB
- Volume
- 273
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
The performance of the recently developed batch inJection analysis (BIA) techmque was investigated with regard to the behavlour of enzyme-unmoblhzed electrodes Glucose oxldase and xanthme ondase sensors were prepared by castmg the enzyme-unmobdlzed membranes on the electrode surface The measurements are based on the amperometric detection of the product of the enzymatic reaction hydrogen peroxide at + 650 mV vs Ag/AgCl (GGx) or the reduced form of methylene blue at +50 mV vs Ag/AgCI (XOD) The enzyme glutamate dehydrogenase was incorporated m the carbon paste and the direct and phenazme methosulphate-medlated detection of NADH was followed at the enzyme electrode The major charactenstlcs observed were sunphc~ty of the equipment, a high sampling rate and hmkxi consumption of the carrrer, I e , reagents such as the mediator and cofactor The repeatability of the manual mJectlon of the sample was shown to be the crltlcal step m the BIA mode A new BIA cell design, allowmg senu-continuous solution drammg, IS reported Keywords AmperometIy, Enzymatic methods, Flow mjectlon, Batch mjectlon analysis, Buxatalytx electrodes, Enzyme electrodes, Glucose, Xanthme Batch mjectlon analysis (BIA) is a recently developed method that consists m mjectmg a sample from a mlcroplpette tip towards a nearby detector mnnersed m a large volume (stirred solution) of a batch wall-jet cell [l] Owmg to the drfflculty of solution handhng m BIA, the use of a detector allowmg high selectlvlty IS of prune 1111portance In this respect, enzyme electrodes may