Investigation of some α-amylases by high-performance hydrophobic-interaction chromatography

✍ Scribed by László Szepesy; László Vida

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 441 KB
• Volume: 499
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96973-5

✦ Synopsis

cl-Amylases of different origins were investigated with a Synchropak propyl column and a linearly descending salt gradient in order to isolate the active fraction from among the inactive proteins present. Fractions collected by several injections were assayed for enzymatic activity by the modified Wohlgemut method. An experimental hydroxypropyl packing was tested for the separation of a-amylases under isocratic conditions, using 'a dilute buffer or plain water as eluent. The chromatograms obtained can serve as fingerprints for the quality control of some industrial proteins.