Investigation of reduced serum and serum-free media for the cultivation of insect cells (Bm5) and the production of baculovirus (BmNPV)
β Scribed by Junli Zhang; Nicolas Kalogerakis; Leo A. Behie; Kostas Iatrou
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 798 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0006-3592
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The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. Sf9 cells in SF90011 medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of lactate and ammonia. Tn5 cells in Excell 401 medi
## Abstract The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt to a multiβparallel process. We have developed a bacmid vector that does not require any for