Investigation of lipase-catalysed hydrolysis of naproxen methyl ester: use of NMR spectroscopy methods to study substrate–enzyme interaction
✍ Scribed by E Cernia; M Delfini; E Di Cocco; C Palocci; S Soro
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 108 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0045-2068
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✦ Synopsis
(+/-)-2-(6-Methoxy-2-naphthyl)propionic acid methyl ester (methyl ester of Naproxen), the precursor of therapeutically important nonsteroidal anti-inflammatory drugs (NSAIDs) was enantioselectively hydrolysed using as biocatalyst Candida rugosa lipase. In research aimed at studying the structure-activity relationship (SAR), NMR spectroscopy methods were employed to identify which Naproxen molecular moiety was essential to the substrate-enzyme interaction. The experimental results, in agreement with previous computer modelling studies and reported kinetic data, gave new information on the enzyme-substrate complex formation in solution.