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Investigation of Enzyme Mechanisms with Nucleoside Phosphorothioates

✍ Scribed by Priv.-Doz. Dr. Fritz Eckstein

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
756 KB
Volume
14
Category
Article
ISSN
0044-8249

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✦ Synopsis

Abstract

Nucleoside phosphorothioates are modified nucleotides in which an oxygen atom bound to a phosphorus atom has been replaced by a sulfur atom. These compounds are suitable for the investigation of enzyme mechanisms because, although they are bound by enzymes in many cases just as well as the natural substrate, the enzymatic reactions very often proceed considerably more slowly. Since nucleoside thiophosphate O‐esters exist as diastereomers these compounds can be used in suitable cases for information about the stereochemistry of enzymatic reactions.

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Theoretical Investigation of the Reactio
Theoretical Investigation of the Reaction Mechanism of the Dinuclear Zinc Enzyme Dihydroorotase
✍ Rong-Zhen Liao; Jian-Guo Yu; Frankβ€…M. Raushel; Fahmi Himo πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 484 KB

## Abstract The reaction mechanism of the dinuclear zinc enzyme dihydroorotase was investigated by using hybrid density functional theory. This enzyme catalyzes the reversible interconversion of dihydroorotate and carbamoyl aspartate. Two reaction mechanisms in which the important active site resid