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Investigation of copper-zinc superoxide dismutase Ser-137 and Ala-137 mutants

โœ Scribed by Banci, Lucia; Bertini, Ivano; Cabelli, Diane; Hallewell, Robert A.; Luchinat, Claudio; Viezzoli, Maria Silvia


Book ID
127330002
Publisher
American Chemical Society
Year
1990
Tongue
English
Weight
693 KB
Volume
29
Category
Article
ISSN
0020-1669

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An investigation of Cu2Zn2superoxide dis
โœ L. Banci; I. Bertini; P. Turano ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 554 KB

The activity profile of the Cu2Zn2HSOD Ile-137 mutant has a pKa of 9.6, i.e. one unit lower than the wild type (WT). This property has allowed us to investigate the inactive high pH form of the enzyme before denaturation occurs. The electronic and EPR spectra do not change with the above pKa. The 1H