𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Investigation of colon cancers for human papillomavirus genomic sequences by polymerase chain reaction

✍ Scribed by Dr. Keerti V. Shah; Richard W. Daniel; Jonathan W. Simons; Bert Vogelstein

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
557 KB
Volume
51
Category
Article
ISSN
0022-4790

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Two previous studies have reported the presence of human papillomavirus (HPV) genomic sequences in colorectal cancers. We examined DNAs from 50 colorectal tumors for HPV sequences by polymerase chain reaction (PCR), using HPV consensus L1 primers for amplification. The PCR products were screened with a generic HPV probe consisting of amplimers of HPV‐16 and HPV‐18. All tumor DNAs were negative for HPV sequences. These results call into the question the reported association of colorectal cancers with HPVs. Β© 1992 Wiley‐Liss, Inc.

πŸ“œ SIMILAR VOLUMES

Amplification of human genomic DNA seque
Amplification of human genomic DNA sequences with polymerase chain reaction using a single oligonucleotide primer
✍ Liuying Luo; Eleftherios P. Diamandis πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 157 KB πŸ‘ 1 views

We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only

Analysis of prostate tissue DNA for the
Analysis of prostate tissue DNA for the presence of human papillomavirus by polymerase chain reaction, cloning, and automated sequencing
✍ Anderson, Michael; Handley, Jane; Hopwood, Lucy; Murant, Susan; Stower, Mike; Ma πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 232 KB πŸ‘ 2 views

zur Hausen and De Villiers, 1994 ]. There has recently We have analysed the DNA from 24 prostate tisbeen an increasing amount of interest in the male genisue biopsies, spanning a range of Gleason gradtalia as both a reservoir for HPV infection and also as a ing from benign to grade 5 and mixed rand

Clonal analysis of human gynecologic can
Clonal analysis of human gynecologic cancers by means of the polymerase chain reaction
✍ Masumi Sawada; Chihiro Azuma; Kazumasa Hashimoto; Shinzaburo Noguchi; Masami Oza πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 French βš– 582 KB

Clonality of human gynecologic cancers was analyzed in small DNA samples prepared from cryostat sections, by means of the polymerase chain reaction (PCR). The method used for clonal analysis was based on restriction fragment length polymorphism of the X-chromosome-linked phosphoglycerokinase (PGK) g

Analysis of deletion of the integrated h
Analysis of deletion of the integrated human papillomavirus 16 sequence in cervical cancer: A rapid multiplex polymerase chain reaction approach
✍ Chuan-Mu Chen; Mei-Pyng Shyu; Lo-Chun Au; Hoi-Weng Chu; Winston T. K. Cheng; Kon πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 740 KB

## Abstract A protocol for a rapid physical mapping of the integrated type 16 human papillomavirus (HPV16) sequences in biospied and paraffin‐embedded archival cervical cancer samples is described. The procedure involves the use of an anchor primer and a mixture of indicator primers in a multiplex

Evaluation of the polymerase chain react
Evaluation of the polymerase chain reaction for the detection of human papillomavirus from urine
✍ James L. Vossler; Betty A. Forbes; Mark D. Adelson πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 732 KB

## Abstract The ability to detect the presence of human pap‐illomavirus (HPV)‐DNA sequences in urine was evaluated using polymerase chain reaction (PCR). DMA was purified and extracted from urine samples, and subjected to 40 cycles of amplification using the consensus primer pair MY11 and MY09. Coa