Investigation of beam-induced reactions occurring under fast-atom bombardment conditions between triethanolamine and various phospholipids
✍ Scribed by Carolien M. Boone; Spiros A. Pergantis; Wigger Heerma
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 159 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
The generation of adduct ions when using a triethanolamine (TEA) matrix under conditions of fast-atom bombardment has been investigated. In particular, phospholipids containing a free amino group react in the TEA matrix to form abundant adduct ions 26 and 42 mass units higher than the analyte pseudomolecular ions. The identity of these adduct ions was investigated using specific phosphatidylethanolamines and phosphatidylserine as well as 2-aminoethyldihydrogenphosphate and 2-aminoethylmonomethylphosphate as model compounds. Evidence obtained from positive-and negative-ion high-energy collision-induced dissociation tandem mass spectra, high resolution mass spectra, deuterium labelling and experiments using diethanolamine/TEA mixtures revealed that the intensities of the adduct ions are dependent on the percentage of TEA in the matrix and on the polarity of the analyte. The nature of the adduct ions is best reflected by a structure in which a hydrogen atom of the free amino group is substituted by a vinyl ( + 26) or by an acetyl ( + 42) group. A mechanism for the adduct ion formation is proposed.