Investigation into the Catalytic Role for the Tryptophan Residues within Domain III of Pseudomonas aeruginosa Exotoxin A †

Application of a Fluorometric Assay for

Application of a Fluorometric Assay for Characterization of the Catalytic Competency of a Domain III Fragment of Pseudomonas aeruginosa Exotoxin A

✍ S. Armstrong; A.R. Merrill 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 113 KB

Pseudomonas aeruginosa exotoxin A (ETA) is a member of the family of bacterial ADP-ribosylating toxins that use NAD(+) as the ADP-ribose donor. The reaction catalyzed by ETA involves the nucleophilic attack of the diphthamide residue on the anomeric carbon of the nicotinamide ribose forming a new gl