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Investigating the specificity of real-time PCR assays using synthetic oligonucleotides

โœ Scribed by N. Boonham; T. Fisher; R.A. Mumford


Book ID
113767087
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
137 KB
Volume
130
Category
Article
ISSN
0166-0934

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Fluorescein-labeled oligonucleotide probes can be used to continuously monitor the polymerase chain reaction. Depending on the sequence, the fluorescence intensity of the probe is either increased or decreased by hybridization. The greatest effect is probe quenching by hybridization to amplicons con