Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method
✍ Scribed by Hana Vaisocherová; Alice Zítová; Markéta Lachmanová; Josef S̆tĕpánek; S̆árka Králíková; Radek Liboska; Dominik Rejman; Ivan Rosenberg; Jir̆í Homola
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2006
- Tongue
- English
- Weight
- 219 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
We have optimized surface plasmon resonance (SPR) biosensor technology for a rapid, direct, and low‐consumption label‐free multianalyte screening of synthetic oligonucleotides (ONs) with modified internucleotide linkages potentially applicable in antisense therapy. Monitoring of the ONs hybridization is based on the formation of complex between the natural oligonucleotide probe immobilized on the sensor surface and the ON in solution in contact with the sensor surface. An immobilization chemistry utilizing the streptavidin–biotin interaction was employed to obtain desired ligand density and high hybridization efficiency. It was demonstrated that the sensor is capable of detecting complementary 23‐mer ONs in concentrations as low as 0.1 n__M__ with high specificity and reproducibility. © 2005 Wiley Periodicals, Inc. Biopolymers 82:394–398, 2006
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