Investigating laser-induced fluorescence of metabolic changes in Guinea pig livers during Nd:YAG laser irradiation
✍ Scribed by O. Minet; J. Beuthan
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 115 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1612-2011
No coin nor oath required. For personal study only.
✦ Synopsis
Recently, dosimetry of thermal influences on biological
tissue has been attracting more and more interest among
scientists. For temperatures exceeding 56°C, the
irreversible tissue coagulation can be approximately described by
the Arrhenius theory, which was established in chemical kinetics.
Investigations in the temperature range between 43°C and
55°C are rare and are, therefore, not available in a
systematic form. A Nd:YAG laser (1060 nm) was used, to heat up
liver tissue of guinea pigs in this temperature range periodically
at intervals of ca. 3 minutes. A second laser (N~2~, 337 nm) was
used to simultaneously excite the autofluorescence of NADH as
coenzyme of the cell respiration. The results of the measurement
series show strong inter-individual variations of the metabolic
decay time. The Arrhenius theory fails to provide a description in
this temperature range thus opening up new modeling possibilities.