✦ LIBER ✦

Intron mutations that affect the splicing efficiency of the CYH2 gene of Saccharomyces cerevisiae

✍ Scribed by Swida, Ulrike ;Thüroff, Eduardo ;Käufer, Norbert F.

Publisher: Springer
Year: 1986
Tongue: English
Weight: 460 KB
Volume: 203
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00333970

No coin nor oath required. For personal study only.

✦ Synopsis

To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing whereas intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.

📜 SIMILAR VOLUMES

Mutations in RAD3, MSH2, and RAD52 affec

Mutations in RAD3, MSH2, and RAD52 affect the rate of gene amplification in the yeast Saccharomyces cerevisiae

✍ Christopher Peterson; Jennifer Kordich; Laura Milligan; Erika Bodor; Angela Sine 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB

Splice variant lacking the transactivati

Splice variant lacking the transactivation domain of the BRCA2 gene and mutations in the splice acceptor site of intron 2

✍ Manuela Santarosa; Alessandra Viel; Mauro Boiocchi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 150 KB 👁 2 views

The sporulation capable (sca) mutation o

The sporulation capable (sca) mutation of Saccharomyces cerevisiae is an allele of the SIR2 gene

✍ Margolskee, Jeanne P. 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 498 KB

We have used the special properties of the spo13-1 mutation in order to study the regulation of yeast meiosis by the mating type loci. We have found that both the rme1-1 mutation and the sca mutation allow haploid meiosis in spo13-1 strains. Therefore, haploid meiosis is regulated in the same manner

A splice junction mutation in intron 2 o

A splice junction mutation in intron 2 of the carbonic anhydrase II gene of osteopetrosis patients from Arabic countries

✍ Peiyi Y. Hu; Donna E. Roth; Laura A. Skaggs; Patrick J. Venta; Richard E. Tashia 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 438 KB

Clinical manifestations in patients with carbonic anhydrase (CA) I1 deficiency include osteopetrosis, renal tubular acidosis, and cerebral calcification. Of the 39 reported cases of the carbonic anhydrase I1 deficiency syndrome, 72% were patients from North African and Middle Eastern countries, most

Mutations of the mitochondrial DNA of Sa

Mutations of the mitochondrial DNA of Saccharomyces cerevisiae which affect the interaction between mitochondrial ribosomes and the inner mitochondrial membrane

✍ Spithill, T. W. ;Trembath, M. K. ;Lukins, H. B. ;Linnane, A. W. 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 796 KB

The Saccharomyces cerevisiae MET3 gene:

The Saccharomyces cerevisiae MET3 gene: Nucleotide sequence and relationship of the 5′ non-coding region to that of MET25

✍ Cherest, Hélène ;Kerjan, Pierre ;Surdin-Kerjan, Yolande 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 689 KB

In Saccharomyces cerevisiae, the expression of several genes implicated in methionine biosynthesis is co-regulated by a specific negative control. To elucidate the molecular basis of this regulation, we have cloned two of these genes, MET3 and MET25. The sequence of MET25 has already been determined