Introduction to Experimental Biophysics, Second Edition: Biological Methods for Physical Scientists

✍ Scribed by Jay L. Nadeau

• Publisher: CRC Press
• Year: 2018
• Tongue: English
• Leaves: 791
• Series: Foundations of Biochemistry and Biophysics
• Edition: Second edition
• Category: Library

✦ Synopsis

Praise for the First Edition

“essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics

"an ambitious text…. Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today

This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions.

Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.

✦ Table of Contents

Content: Basic molecular cloning of DNA and MA --
Expression of genes in bacteria, yeast, and cultured mammalian cells --
Advanced topics in molecular biology --
Protein expression methods --
Protein crystallization --
Introduction to biological light microscopy --
Advanced light microscopy techniques --
Advanced topics in microscopy II: holographic microscopy --
Quantitative cell culture techniques --
Semiconductor nanoparticles (quantum dots) --
Gold nanoparticles --
Advanced topics in gold nanoparticles: biomedical applications --
Surface functionalization techniques --
Electrophysiology --
Spectroscopy tools and techniques --
Introduction to nanofabrication.

✦ Subjects

Biophysics -- Experiments -- Technique.