Introduction of wild-type p53 gene downregulates the expression of H-ras gene and suppresses the growth of bladder cancer cells

✍ Scribed by Li, M. ;Gu, F. L. ;Li, W. B. ;Song, Y. S. ;Zhou, A. R. ;Guo, Y. L.

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 460 KB
• Volume: 23
• Category: Article
• ISSN: 0300-5623
• DOI: 10.1007/bf00300019

✦ Synopsis

Retroviral vectors were used to introduce the wild-type p53 gene into human bladder cancer cell lines BIU-87 and EJ, which express endogenous wt-p53 gene and have a mutation in H-ras gene. The expression of the exogenous wt-p53 gene in cells suppresses the growth of the bladder cancer cells in standard culture and in soft agar and blocks the cell cycle progression in G1. The BIU-87 and EJ cells developed tumors with average volumes of 6.53 cm3 and 6.61 cm3 in nude mice in 9 weeks after inoculation, while the cells transduced with wt-p53 gene failed to form tumors. The expression of H-ras gene in bladder cancer cells was reduced at mRNA level. These results suggest that the overexpression of the wt-p53 gene suppresses the expression of mutant H-ras gene and inhibits the tumor cell growth in vivo and in vitro.