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Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene

✍ Scribed by Thomas, Kirk R.; Capecchi, Mario R.


Book ID
109747416
Publisher
Nature Publishing Group
Year
1986
Tongue
English
Weight
610 KB
Volume
324
Category
Article
ISSN
0028-0836

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The mutagenic effect of papovavirus SV40 on human cells could be demonstrated by reversion of HPRT deficiency in Lesch-Nyhan fibroblasts transformed by the virus. SV40 seems to induce different gene mutations in individually selected cell clones, as was clearly shown by the respective HPRT enzyme pr