Introduction of Functional Groups onto Polypropylene and Polyethylene Surfaces for Immobilization of Enzymes
β Scribed by Azmi Naqvi; Pradip Nahar; R.P. Gandhi
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 55 KB
- Volume
- 306
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Polypropylene and polyethylene surfaces are activated by introducing an active functional group through 1-fluoro-2 nitro-4-azidobenzene by UV irradiation. Horseradish peroxidase and glucose oxidase are immobilized onto the activated surfaces, simply by incubating the enzymes at 37Β°C. When untreated surfaces are used, insignificant immobilization of the enzymes is observed.
π SIMILAR VOLUMES
Anhydrous ammonia gaseous plasma technique was used for the surface modification of polypropylene beads. Amino groups were added onto the surfaces of beads by exposing them to ammonia plasma. Through these amino groups covalent immobilization of glucose oxidase and peroxidase were carried out. The t