Introduction of Fluorometry to the Screening of Protein Crystallization Buffers
β Scribed by Takamitsu Ikkai; Katsuhiko Shimada
- Book ID
- 110354680
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 52 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1053-0509
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Macromolecular crystallization remains a bottleneck in structure determination by X-ray diffraction. Based on the data reflecting success rates of crystallization conditions in different screens and the information derived from the BMCD and other related studies, a simplified screen has been designe
Selective crystallization of buffer components in frozen solutions is known to cause pronounced pH shifts. Our objective was to study the crystallization behavior and the consequent pH shift in frozen aqueous carboxylic acid buffers. Aqueous carboxylic acid buffers were cooled to -25Β°C and the pH of