Intrinsic42Ca-Labelling of Green Bean Pods for Use in Human Bioavailability Studies
✍ Scribed by Grusak, Michael A; Pezeshgi, Shahrbanu; O’Brien, Kimberly O; Abrams, Steven A
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 516 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
✦ Synopsis
A hydroponic growth procedure which enables the intrinsic 42Calabelling of green beans (Phaseolus vulgaris L) has been developed. Whole-plant Ca influx was measured from planting through the period of pod expansion for a crop of 60 plants grown hydroponically in a 3 m2 area. Total Ca influx was 10.1 mmol per plant; 35% of this influx occurred during the period of pod growth. Plants were labelled with 42Ca via the nutrient solution during the period of pod expansion; isotopic composition of harvested pods was nearly identical to that of the administered solution. Recovery of isotopic tracer at harvest was as follows: pods 8.4%; leaves, 61.1%; stems, 17.1%, roots, 9.0%. Pod yield was 98.8 g FW per plant. 42Ca-labelled green beans can be used safely to investigate Ca bioavailability in humans.