The role of Asp-49 and other conserved a
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Carel J. van den Bergh; Arend J. Slotboom; Hubertus M. Verheij; Gerard H. de Haa
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Article
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1989
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John Wiley and Sons
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English
β 778 KB
The role of aspartic acid49 (Asp-49) in the active site of porcine pancreatic phospholipase A2 was studied by recombinant DNA techniques: two mutant proteins were constructed containing either glutamic acid (Glu) or lysine (Lys) at position 49. Enzymatic characterization indicated that the presence