Intraphagolysosomal pH in Canine and Rat Alveolar Macrophages: Flow Cytometric Measurements
โ Scribed by Peter Heilmann, Wolfgang Beisker, Ursula Miaskowski, Per Camner and Wolfgang G. Kreyling
- Book ID
- 124310964
- Publisher
- Environmental Health Information Service
- Year
- 1992
- Tongue
- English
- Weight
- 448 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0091-6765
- DOI
- 10.2307/3431338
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๐ SIMILAR VOLUMES
In the article by Kharbanda et al. (Flow Cytometric Analysis of Vesicular pH in Rat Hepatocytes After Ethanol Administration'' Vol. 26, No. 4,, the second line in the legend to Figure 4 reads ''25 ยตmol/L ethanol.'' The correct unit of measure is ''25 mmole/L ethanol.''
We examined the effect of ethanol administration on intravesicular pH in intact hepatocytes by applying a flow cytometric technique to detect fluorescein-isothiocyanate-dextran (FITC-dextran) in acidic vesicles. Rats were pair-fed liquid diets containing either ethanol or isocaloric carbohydrate for
We describe a new method for calibrating intracellular pH (pH,) measurements by flow cytometry, based on the null point method proposed originally by Eisner et al. (Pflugers Arch 413:55+558, 1989). The method involves suspending cells loaded with pH-sensitive dyes, such as SNARF-1 or BCECF, in deh