✦ LIBER ✦
Intramolecularly quenched fluorogenic tetrapeptide substrates for tissue and plasma kallikreins
✍ Scribed by Jair R. Chagas; Luiz Juliano; Eline S. Prado
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 786 KB
- Volume
- 192
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Five intramolecularly quenched fluorogenic substrates for arginyl hydrolases with the sequence Abz-Phe-Arg-X-Y-EDDnp (X = Arg or Ser; Y = Val, Pro, or Arg) were synthesized by classical solution methods. Kinetics of their hydrolysis by tissue and plasma kallikreins, trypsin, and thrombin characterized Abz-Phe-Arg-Ser-Arg-EDDnp as a specific and sensitive substrate for the continuous assay of tissue kallikreins while Abz-Phe-Arg-Arg-Pro-EDDnp was the best substrate for human plasma kallikrein. The five peptides were poor substrates for trypsin and resistant to thrombin.