Intracytoplasmic membranes in oxygen-limited chemostat cultures ofMethylosinus trichosporiumOB3b: Biocatalytic implications of physiologically balanced growth
✍ Scribed by D. Scott; D. J. Best; I. J. Higgins
- Publisher
- Springer Netherlands
- Year
- 1981
- Tongue
- English
- Weight
- 221 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
The continuous culture growth conditions for induction of intracytoplasmic membranes in Methylosinus trichosporium OB3b are described. During oxygen-limited, nitrate-excess chemostat culture, organisms have an extensive intracytoplasmic membrane system and particulate, cell-free methane mono-oxygenase (MMO). Under methane limitation fewer intracytoplasmic membranes are seen, while under all other conditions tested, membranes are absent and cell-free MMO is entirely soluble. These findings may be important in relation to the development of oxidative biotransformation processes using this bacterium.