Intracranial infusion of monoamine-activated α2-Macroglobulin decreases dopamine concentrations within the rat caudate putamen
✍ Scribed by Y.-Q. Hu; D. E. Dluzen; P. H. Koo
- Book ID
- 102910697
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 722 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Monoamine-activated a,-macroglobulin (a2M) has been shown to inhibit choline acetyltransferase in basal forebrain neurons as well as neurotrophin-dependent neuronal functions. The objective of this study was to determine whether monoamine-activated a,M can affect the caudate putamen (CP) dopaminergic system in vivo. Male rats received intracranial infusions of methylamine-activated a,M (0.6 nmole) and contralateral infusions of its vehicle, phosphate-buffered saline (PBS). Five days following infusion, the animals were killed, the CP dissected into three rostral-caudal segments, and assayed for dopamine (DA) using a high-performance liquid chromatography system. Within the two rostra1 CP segments (the approximate site of cannula placement), statistically significant (26%) reductions of DA concentrations were obtained on the a,M-infused side of the CP with 90-100% of the animals showing decreases. At a more distal (caudal) site of the CP, DA concentrations showed only an insignificant (12%) reduction. No differences in DA concentrations between sides infused with bovine serum albumin versus PBS or from olfactory tubercle samples were obtained in these animals. These results demonstrate that monoamine-activated a,M is capable of producing significant degeneration of the nigrostriatal dopaminergic system in vivo and suggest that this factor may play a role in age-related neurodegenerative disorders such as Parkinson's disease.