The present study describes the cell volume dynamics in intact rat hearts, during ischemia and after reperfusion. Cell volumes were measured in isolated hearts by either 13C or 5aC0 NMR of mannitol or cobalticyanide, respectively, as extracellular markers and 'H NMR of water as the aqueous space mar
Intracellular volume of rat hearts and oedema during ischemia- 1H and Co59 NMR studies
โ Scribed by Nadir Askenasy; Maria Tassini; Antonio Vivi; Gil Navon
- Book ID
- 115984854
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 103 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0022-2828
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๐ SIMILAR VOLUMES
A spin-echo method is presented for obtaining high resolution, '"C coupled, proton spectra of lactate and alanine in intact, beating rat hearts. All hearts were depleted of glycogen prior to prolonged perfusion with either 10 mM unenriched glucose or [l-'3C]glucose to restore glycogen. These two gro
The present study describes an experimental setup that enables continuous measurement of cellular volumes in isolated organs. The procedure is a modification of a recently reported method that uses multinuclear NMR measured by 59Co NMR of cobalticyanide and 1H NMR of water in isolated rat hearts at