Intracellular pH, cytosolic calcium concentration and electrical activity in RINm5F insulinoma cells
β Scribed by L. Best; C. Ammala; P. Rorsman; S. Tomlinson
- Book ID
- 115730773
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 397 KB
- Volume
- 1192
- Category
- Article
- ISSN
- 0005-2736
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By using a FURA2 ratio imaging method, the intracellular free calcium concentration was investigated in cultured mice neuroblastoma cells under the influence of an amplitude-modulated (AM) field (5 kHz sine wave AM 16 Hz sinusoidal 800 V/m and 80 V/m), as well as of electric field pulses (300-ms uni
Thyrotropin-Releasing hormone (TRH)-degrading pyroglutamyl peptidase I (PGP I) and prolylendopeptidase (PE) activities have been demonstrated in rat insulinoma RINm 5F cell line. These two enzymes catalyze the conversion of TRH to Histydyl-Proline-Diketopiperazine and to acid TRH respectively. After