Intracellular pH and cytoplasmic free Ca2+
โ Scribed by Siffert, W.; Akkerman, J. W. N.
- Book ID
- 109749614
- Publisher
- Nature Publishing Group
- Year
- 1987
- Tongue
- English
- Weight
- 145 KB
- Volume
- 327
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/327376a0
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In cells of Zea mays (root hairs, coleoptiles) and Ricciafluitans (rhizoids, thalli) intracellular Ca 2 + and pH have been measured with doublebarrelled microelectrodes. Free Ca 2+ activities of 109-187 nM (Riccia rhizoids), 94 160 nM (Riccia thalli), 145-231 nM (Zea root hairs), 84-143 nM (Zea cole
The transformation of certain cells reduces the requirement of extracellular Ca'+ for growth. The SV-40 transformed human lung fibroblasts, WI-38 VA13, require less Ca" than normal WI-38 cells. Spreading area of the normal cells decreases when cultured in 1 0 p M Ca2+ medium. lntracellular calcium c