Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins

✍ Scribed by Gary A. Clawson; Vuong Bui; Ping Xin; Ning Wang; Weihua Pan

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 321 KB
• Volume: 105
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.21804

✦ Synopsis

Abstract

We have a long‐standing interest in a nuclear protease which appears to be involved in carcinogenesis. We recently identified the protease as high temperature requirement factor A 1 (HtrA1), also known as Prss11, which is member of an oxidative stress‐response family of proteases. HtrA1 has been classified as a secreted protease involved in TGFβ signaling, but recent work has shown HtrA1 to be a tumor suppressor. Here we show that processed forms of HtrA1 are found intracellularly and intranuclearly, and the active intranuclear form of HtrA1 shows an ∼Mr 29,000. Further, expression of HPV E6/E7 proteins is associated with a post‐transcriptional up‐regulation of HtrA1 (most notably the nuclear form), and HtrA1 is found associated with both HPV E6 and E7 proteins. J. Cell. Biochem. 105: 81–88, 2008. © 2008 Wiley‐Liss, Inc.