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Intracellular localization of glutamine synthetase inChlorogloeopsis fritschii

✍ Scribed by A-K.J. Sallal

Publisher: Springer
Year: 1994
Tongue: English
Weight: 406 KB
Volume: 10
Category: Article
ISSN: 1573-0972
DOI: 10.1007/bf00144457

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✦ Synopsis

After differential centrifugation of cell-free extracts of Chlorogloeopsis fritschii, 71% of the original glutamine synthetase (GS) activity was associated with the thylakoids, while little activity was detected in the cytoplasmic membranes. Monospecific antiserum to a purified GS inhibited 88% of the enzyme activity in solubilized thylakoid membranes. An antiserum raised against thylakoids gave 81% inhibition. However, using intact thylakoid membranes, only 7% inhibition was obtained with the GS antiserum, indicating that GS is located inside the thylakoid membranes.

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