Intracellular calcium oscillations in a T-cell line after exposure to extremely-low-frequency magnetic fields with variable frequencies and flux densities
✍ Scribed by Ewa Lindström; Per Lindström; André Berglund; Erik Lundgren; Kjell Hansson Mild
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 515 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0197-8462
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✦ Synopsis
Low-frequency magnetic fields (MF) can increase the cytosolic calcium concentration ([Ca'+]) in lymphocytes in the same manner as a physiological stimulus such as antibodies directed towards the CD3 complex. In this study, MF with various frequencies and flux densities were used, while [Ca'+], changes were recorded using microfluorometry with fura-2 as a probe. The applied sinusoidal M F induced oscillatory changes of [Ca"], in the leukemic cell line Jurkat in a manner similar to that seen with stimulation by antibodies. The response at 0.15 mT was over a frequency range from 5 to 100 Hz, with a fairly broad peak having its maximum at 50 Hz. The result of testing increasing flux densities at 50 HI. was a threshold response with no effect below 0.04 mT and a plateau at 0.15 mT. On the basis of the characteristic calcium pattern resulting from an applied MF, we suggest that MF influence molecular events in regular signal transduction pathways of T cells.