✦ LIBER ✦

Intracellular calcium chelators and oxidant-induced renal proximal tubule cell death

✍ Scribed by Schnellmann, Rick G.

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 308 KB
Volume: 6
Category: Article
ISSN: 0887-2082
DOI: 10.1002/jbt.2570060410

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✦ Synopsis

The effect of intracellular calcium chelators on rabbit renal proximal tubule (RPT) cell death induced by t-butyl hydroperoxide (TBHP) and H,O, was examined. Preincubation of RPT suspensions with 50 pM QUIN 21AM completely prevented TBHP (0.5 mM) and H,O, (2 mM) induced cell death [i.e., release of lactate dehydrogenase (LDH)]. QUIN 2/AM, BAPTAIAM, EGTAIAM, and FURA 21AM, at 5 pM, decreased LDH release (at 6 hr) from 41% to 4%, 21%, 26% , and 33% , and decreased lipid peroxidation (at 1 hr) from 1.0 to 0.1, 0.4, 0.6, and 0.8 nmol MDAlmg protein, respectively, after TBHP exposure. Since oxidant-induced lipid peroxidation and cell death are iron-dependent in this model, these results suggest that the intracellular calcium chelators inhibit cell death by chelating iron.

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