✦ LIBER ✦

Intracellular calcium as a second messenger for human hepatocyte growth factor in hepatocytes

✍ Scribed by Akira Kaneko; Norio Hayashi; Hirohito Tsubouchi; Yuji Tanaka; Toshifumi Ito; Yutaka Sasaki; Hideyuki Fusamoto; Yasushi Daikuhara; Takenobu Kamada

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 645 KB
Volume: 15
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840150632

No coin nor oath required. For personal study only.

✦ Synopsis

Human hepatocyte growth factor is a newly discovered substance that stimulates DNA synthesis in vitro. In this study, we examined intracellular Ca2+ movement as one of the second messengers for human hepatocyte growth factor in primary-cultured hepatocytes. The addition of hHGF induced Ca2+ oscillation, but the frequency of oscillations varied from cell to cell. We also saw marked intercellular heterogeneity in the initial latent period for the Ca2+ response; the mean latent period was rather longer than those seen with phenylephrine and vasopressin. This difference in the initial latent period may be due to the difference in the pathways of Ca2+ elevation. Duration of culture determined the number of human hepatocyte growth factor-responsive cells; their number peaked at 2 to 5 hours of confluent culture, whereas the peak was earlier in a low-density culture. These changes in responsiveness during culture can be explained by the cell cycle-dependent sensitivity to human hepatocyte growth factor of hepatocytes. The Ca2+ response to human hepatocyte growth factor was dose dependent; 10(-10) mol/L hHGF gave the highest Ca2+ response, similar to the dose-response curve of DNA synthesis. We even observed the Ca2+ response in the Ca(2+)-free buffer, so the increase in Ca2+ was considered due to release from intracellular Ca2+ stores. These results suggest that human hepatocyte growth factor causes the intracellular Ca2+ elevation in the early stage of the cell cycle and that it plays important roles in the signal transduction systems for human hepatocyte growth factor and the proliferation of hepatocytes.

📜 SIMILAR VOLUMES

Expression of hepatocyte growth factor,

Expression of hepatocyte growth factor, transforming growth factor α, and transforming growth factor β 1 messenger RNA in various human liver diseases and correlation with hepatocyte proliferation

✍ M Masuhara; M Yasunaga; K Tanigawa; F Tamura; S Yamashita; I Sakaida; K Okita 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB

After a partial hepatectomy, TGF-a mRNA has been shown of chronic liver disease; CH, chronic hepatitis; LC, liver cirrhosis; LI, labeling index; RTto increase in hepatocytes at the time of DNA synthesis con-PCR, reverse-transcriptase polymerase chain reaction; cDNA, complementary DNA.

Mammalian hepatocytes as a foundation fo

Mammalian hepatocytes as a foundation for treatment in human liver failure

✍ Hugo O. Jauregui; Kathryn L. Gann 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 717 KB

Technological advances in the separation and culture of mammalian hepatocytes have facilitated the use of these cells as the foundation for either hepatocyte transplantation or hepatocyte-seeded hollow fiber liver assist devices (LAD). To fully appreciate the practical applications of these tissue e

Effects of hepatocyte growth factor (HGF

Effects of hepatocyte growth factor (HGF) on human glioma cells in vitro: HGF acts as a motility factor in glioma cells

✍ Takuzou Moriyama; Hiroaki Kataoka; Kohji Seguchi; Hirohito Tsubouchi; Masashi Ko 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 996 KB

Expression of c-Met, the receptor for hepatocyte growth factor (HGF). and the biological roles of HGF were examined in cultured human glioma cells. All of the 5 glioma cell lines examined expressed c-Met protein as well as the c-met gene. Expression of the c-met gene was also confirmed in a glioblas

IMMUNOREACTIVITY FOR HEPATOCYTE GROWTH F

IMMUNOREACTIVITY FOR HEPATOCYTE GROWTH FACTOR/SCATTER FACTOR AND ITS RECEPTOR, met, IN HUMAN LUNG CARCINOMAS AND MALIGNANT MESOTHELIOMAS

✍ HARVEY, P.; WARN, A.; NEWMAN, P.; PERRY, L. J.; BALL, R. Y.; WARN, R. M. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 756 KB

Paraffin sections from 29 lung carcinomas (28 primary and 1 metastatic) and 9 pleural malignant mesotheliomas were immunostained with antisera to human hepatocyte growth factorlscatter factor (HGFISF) and its receptor, met. For HGFISF, immunoreactivity was demonstrated in all 9 mesotheliomas, 9 of 1

Serum hepatocyte growth factor as a prog

Serum hepatocyte growth factor as a prognostic marker for stage II or III colorectal cancer patients

✍ Yuji Toiyama; Chikao Miki; Yasuhiro Inoue; Yoshinaga Okugawa; Kouji Tanaka; Masa 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 205 KB

## Abstract We previously reported that a combination of serum hepatocyte growth factor (HGF) and carcinoembryonic antigen (CEA) was useful for selecting early‐stage colorectal cancer patients with aggressive disease. The aim of the present study was to determine whether serum HGF could provide CEA

Direct evidence that hepatocyte growth f

Direct evidence that hepatocyte growth factor is a hepatotrophic factor for liver regeneration and has a potent antihepatitis effect in vivo

✍ Yoshihide Ishiki; Hiroo Ohnishi; Yasutoshi Muto; Kunio Matsumoto; Toshikazu Naka 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 987 KB

Hepatocyte growth factor, a potent mitogen for mature hepatocytes in vitro, seems to function as a hepatotrophic factor for liver regeneration. We examined the mitogenic effect of hepatocyte growth factor on mouse liver in vivo. The labeling i n k of hepatocytes was markedly increased when recombina