Intracellular Ca2+ release mediates ursolic acid–induced apoptosis in human leukemic HL-60 cells

✍ Scribed by Jin Hyen Baek; Yong Soo Lee; Chang Mo Kang; Jung-Ae Kim; Kyung Sool Kwon; Han Chul Son; Kyu-Won Kim

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: French
• Weight: 86 KB
• Volume: 73
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19971127)73:5<725::aid-ijc19>3.0.co;2-4

✦ Synopsis

The effect of ursolic acid (UA) on tumor cell apoptosis was investigated using HL-60 human promyelocytic leukemia cells as a model cellular system. Treatment with UA resulted in a concentration-dependent decreased cell viability assessed by MTT assay. UA also induced genomic DNA fragmentation, a hallmark of apoptosis, indicating that the mechanism by which UA induced cell death was through apoptosis. The intracellular Ca2+ level was increased by treatment with UA. Intracellular Ca2+ inhibitors, such as intracellular Ca2+-release blockers (dantrolene, TMB-8 and ruthenium red) and an intracellular Ca2+ chelator (BAPTA/AM), significantly blocked the UA-induced increased intracellular Ca+ concentration. These inhibitors also blocked the effects of UA on cell viability and apoptosis. These results suggest that enhanced intracellular Ca2+ signals may be involved in UA-induced apoptosis in HL-60 cells.