✦ LIBER ✦
Intracellular acidification mediates the proliferative response of PC12 cells induced by potassium ferricyanide and involves MAP kinase activation
✍ Scribed by Didier Thomas; Marie-Françoise Ritz; Anant N. Malviya; Stéphane Gaillard
- Book ID
- 102654661
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 769 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Potassium ferricyanide is known to elicit cell growth and mitogenesis in various cells by stimulating a transplasma membrane electron-transport system. When serum-starved PC I 2 cells were treated with potassium ferricyanide, stimulation of mitogenesis was evidenced by enhanced DNA synthesis, as well as by increased cell numbers. lntracellular pH @Hi) of PCl2 cells was measured at 37°C by microfluorimetric analysis of 2',7'-bis-(2sarboxyethyl)-5(and -6)-carboxyRwrexein (BCECF).
The resting pHi of unstimulated cells was 7.52 (external pH