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Intestinal absorption pathway of γ-aminobutyric acid in rat small intestine

✍ Scribed by A. Nácher; A. Polache; M. J. Moll-Navarro; J. M. Plá-Delfina; M. Merino

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
630 KB
Volume
15
Category
Article
ISSN
0142-2782

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✦ Synopsis

Intestinal absorption of y-aminobutyric acid (GABA), as a model compound for y-aminoacids, has not been extensively studied from the kinetic viewpoint. Since data from our laboratory suggested that some competition arises between intestinal absorption of palanine and GABA and since our intent was to maintain the aqueous stagnant diffusion layer in order to approach absorption tests to in vivo physiological conditions, a rat jejunum in situ study was undertaken in order to gain an insight into the mechanism of GABA absorption. In the present paper, results from assays using isotonic perfusion solutions with starting GABA concentrations ranging from 1 to 50mM are reported. They show that the intestinal absorption of the y-aminoacid can be apparently described as a specialized transport mechanism which obeys Michaelis-Menten and first-order kinetics. Parameter values found were V, = 13 -99 2 2-37 mM h-I, Km=3-87?0.63mM, and ka(passive)=0.362+0-120h-'. Through the perfusion of 5 mM O-alanine solutions containing variable concentrations of GABA (from 5 to 50mM), a partially competitive inhibition of /3-alanine absorption was apparently characterized.

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