Interspecific hybrids ofCucumis metuliferus × C. anguriaobtained through embryo culture and somatic embryogenesis
✍ Scribed by George Fassuliotis; B. V. Nelson
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 660 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
✦ Synopsis
Interspecific crosses between Cucumis metuliferus Naud . and C. anguria L . were obtained through embryo culture . Embryos in the rabbit-ear to advanced fluke-shaped stages were rescued 34-99 days after pollination . Plants were obtained through direct embryo culture, and through somatic embryogenesis from immature embryos . For direct embryo culture, fluke-shaped embryos were stored in sterile water in darkness for three days at 25C prior to transfer on Murashige and Skoog (MS) culture medium plus 1 .0µM 6-benzylamino-purine . Multiple plants were obtained from single embryos through somatic embryogenesis of rabbit-ear stages on MS plus 10 µM indole-3-acetic acid and 5 µM 6-benzylamino-purine . Evidence of hybridization included leaf shape intermediate between the two parents, penduncle shape prior to fertilization which resembled the male parent, low pollen viability and isoelectric focussing of protein bands for acid phosphatase of leaf extracts .