Hybrids were obtained from Ipomoea interspecific crosses through ovule culture. The hybridity of the progeny obtained from L triloba x I. trifida and (L triloba x I. lacunosa) ร I. batatas (4x) crosses was established by comparisons of floral morphology and analyses of peroxidase and esterase isozymes. The hybrids displayed the inflorescence type and sepal shape and texture of their male parents, while corolla size and anther and nectary color tended to be intermediate to their parents. The isozyme banding patterns of the hybrids contained bands present in the patterns of each of their parents. Pollen grain viability, measured by aceto-carmine stainability, was 44.1%, 92.3% and 82.4%, respectively, for the 1. triloba x I. trifida hybrid and the (I. triloba x L lacunosa) x I. batatas (4x) hybrids, HI and H2. A controlled pollination study revealed that the L triloba x I. trifida, and the (I. triloba ร I. lacunosa) x I. batatas (4x) hybrids, Hi and H2 were partially self fertile with 6%, 70% and 13%, respectively, of the pollinated flowers producing viable seed. Success in backcrossing and sib-mating varied with the cross combination.