Interspecies exchange ofβ2-microglobulin and associated MHC and differentiation antigens

✍ Scribed by Wilhelm Schmidt; Hilliard Festenstein; Philip J. Ward; Arnold R. Sanderson

• Book ID: 104731710
• Publisher: Springer-Verlag
• Year: 1981
• Tongue: English
• Weight: 573 KB
• Volume: 13
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00343716

✦ Synopsis

Radiolabeled human beta 2-microglobulin (beta 2m) can bind to mouse histocompatibility (H-2) antigens on the cell surface or to partially purified H-2 antigens in solution. The complexes containing human beta 2m and H-2 antigens from C3H (H-2k) mice could be immunoprecipitated specifically with alloantisera, rabbit anti-H-2 xenoantisera, and with monoclonal H-2-specific antibodies. Specific association with H-2 antigens was also observed with other haplotypes. The only exception was B10.D2 (H-2d) from which complexes containing human beta 2m could only be precipitated with anti-H-2-xenosera. Thus radiolabeled human beta 2m can be used as a specific label for mouse H-2 antigens in precipitation and radioimmunoassays. The application of this finding extends to major histocompatibility complex antigens of other species, and to differentiation antigens with primary association with beta 2m.