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Interrelationships among X-ray-induced anchorage independence, mutagenesis and chromosomal rearrangements in human diploid fibroblasts

✍ Scribed by Yoshio Kano; Andrew J. Grosovsky; John B. Little


Publisher
John Wiley and Sons
Year
1987
Tongue
French
Weight
588 KB
Volume
40
Category
Article
ISSN
0020-7136

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✦ Synopsis


The changes in the frequencies of X-ray-induced mutations to 6-thioguanine resistance. chromosomal abnormalities and transformation to anchorage-independent growth were examined during confluent holding recovery in density-inhibited cultures of human diploid fibroblasts. Chromosomal abnormalities studied included non-stable aberrations, deletions, and reciprocal translocations measured up to 20 mean population doublings post-irradiation. Complex recovery kinetics were observed. A comparison of these kinetics suggests that reciprocal translocations are associated with the induction of both transformation and mutations. Mutagenesis correlated with deletions and translocations, whereas transformation correlated with translocations only.