Interpretation of the real plate number concept: A fundamental analysis

✍ Scribed by Smuts, T. W. ;Buys, T. S. ;de Clerk, K. ;du Tolt, T. G.

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 364 KB
• Volume: 1
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240010109

✦ Synopsis

The real plate number concept is interpreted in terms of the fundamental properties of a chromatograph. It is shown that the linear relationship between the total peak width at half height, (bo.s)~,t,and kisonly anapproximation of the more general linear relationship between (bo.5)2~, t and (1 + k)2. In the case where the relationship between (b0.5) T, t and k approaches linearity, it is concluded, firstly, that bo = a, i. e., bo is a column property and independent of extra-column phenomena and, secondly, that nreal = ntheor. When the relationship becomes non-linear, bo is determined partly by extra-column phenomena, but not in terms of a useful relationship. It is concluded that it is unnecessary to introduce the parameters a, bo and nreal, since they all have counterparts in the established theory of chromatography. These counterparts prove to be equally useful in the practical analysisof chromatographic systems.

appears to be well established [3.4] that, with each of these categories, there is associated a specific contribution to @T, t -02e, t and oZc, t for the extra-column and column