Interpretation of equilibrium sedimentation measurements of proteins in guanidine hydrochloride solutions. Partial volumes, density increments, and the molecular weight of the subunits of rabbit muscle aldolase

A method for the measurement of the sedi

A method for the measurement of the sedimentation coefficient and molecular weight of microgram quantities of proteins in 6 m guanidine hydrochloride

✍ Bruce W. Patterson; Verne N. Schumaker; Waldo R. Fisher 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 421 KB

A technique has been perfected for measuring the sedimentation coefficient of microgram quantities of a reduced protein in 6 M guanidine hydrochloride. The protein is sedimented through a gradient of 5-8 M guanidine-HCl in the presence of dithiothreitol in a SW 50.1 swinging-bucket rotor. Run condit