Interpretation of electrospray and atmospheric pressure chemical ionization mass spectra of 10-formyl-7,8-dihydrofolic acid and 5-formyl-5,6,7,8-tetrahydropteroic acid
✍ Scribed by Michal Holčapek; Pavel Jandera
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 66 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
Interpretation of positive-and negative-ion electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) mass spectra of 10-formyl-7,8-dihydrofolic acid (10-FDHFA) and 5-formyl-5,6,7,8-tetrahydropteroic acid (5-FTHPA) is discussed. ESI mass spectra enable unambiguous molecular weight (MW) determination. In addition to the determination of MW, APCI mass spectra also make possible structure elucidation of 10-FDHFA and 5-FTHPA. ESI and APCI are complementary ionization techniques and appear to be useful alternatives to conventional electron ionization (EI) for the structure elucidation of non-volatile carboxylic acids. Prior to mass spectral analysis, the acids investigated were separated by reverse-phase high-performance liquid chromatography (HPLC).